Natura Montenegrina No 4, 2005
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Natura Montenegrina No 4, 2005 |
THE
EFFECT OF PLANT GROWTH PROMOTING RHIZOBACTERIA ON SOME PHYSIOLOGICAL PROCESSES
OCCURING DURING THE IN VITRO GERMINATION
OF MAIZE (ZEA MAYS L.) CARYOPSES
Stefan
MARIUS1, Ailiesei OCTAVITA2, Erica
NIMITAN3, Simona DUNCA4,
Magdalena ZAMFIRACHE 5, Eugen
UNGUREANU6
S y n o p s i s
Our
efforts were pointed out to the influence of some rhizobacterial strains on the
starch content of Zea mays L.
caryopses and some energy-generating processes during in vitro germination. α-amylase maximum activity was recorded
for the test in the 12th day of germination and for the control in
the 11th day. During the entire in
vitro germination of maize caryopses, α-amylase activity was
characterized by an increase in intensity, not only in the test lot, but also in
the control one. The dynamics of the catalase and acid phosphomonoesterase
activities, both for the test and for the control, follows the evolution
of the respiratory processes during the germination monitored in laboratory
conditions.
In the first part of germination, catalase activity of the test lot
proved more intense than that of the control lot, as a response to the
biological stress due to the influence of rhizobacteria.
With the appearance of radicles, the response to the biological stress
diminishes, as a sign of the adaptation of germinated caryopses to the
inoculated microorganisms.
Key words: Zea mays, germination, plant growth promoting rhizobacteria, α-amylase,
catalase, acid phosphomonoesterase.
S
i n o p s i s
UTICAJ RIZOBAKTERIJA KOJE POSPJEŠUJU RAST BILJAKA NA NEKE FIZIOLOŠKE
PROCESE KOJI SE JAVLJAJU TOKOM KLIJANJA KUKURUZA (ZEA MAYS L.) IN VITRO
Naši napori su bili upravljeni na uticaj nekih
rizobakterijskih vrsta na sadržaj skroba kod zrna Zea mays L. i neke
procese stvaranja energije tokom klijanja in vitro. Maksimalna aktivnost α-amilaze zabilježena je za test 12. dana klijanja, a za kontrolu
11. dana. Tokom čitavog klijanja in vitro zrna kukurza, aktivnost
α-amilaze karakterisala se povećanjem intenziteta, ne samo na probi,
već i na kontroli. Dinamika aktivnosti katalaze i acido fosfomonoesteraze,
kako za test tako i za kontrolu, prati razvoj respiratornih procesa tokom
klijanja praćenog u laboratorijskim uslovima. U prvom dijelu klijanja,
aktivnost katalaze testa pokazala se intenzivnijom nego što je bio slučaj
kod kontrole, kao odgovor na biološki stres uslovljen uticajem rizobakterija.
Pojavom radikala, odgovor na biološki stres se smanjuje, kao znak adaptacije
isklijalih zrna na inokulirane mikroorganizme.
Ključne riječi: Zea mays, klijanje, rizobakterije koje pospješuju rast biljke, α-amilaza,
katalaza, acid fosfomonoesteraza.
_____________________________
1,2,
3, 4, 5, 6
University
“Al.I.Cuza”, Iasi”, Faculty of Biology, Microbiology Department, B-dul
Carol I, 11A 700506, Iasi-România. Corresponding author: Stefan Marius, PhD
student (e-mail: stefanm@uaic.ro)